abstract：:Destruxins have been implicated in the infection process by entomopathogenic fungi and have been also found to be highly toxic when applied topically or ingested by different insect species. To gain insight into the mechanism of action of this toxin on insect internal organs, we have evaluated the effects of destruxin...

journal_title：Archives of insect biochemistry and physiology

authors： Ruiz-Sanchez E,O'Donnell MJ

更新日期：2012-07-01 00:00:00

abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...

journal_title：Archives of insect biochemistry and physiology

authors： Sun Z,Yu J,Wu W,Zhang G

更新日期：2012-06-01 00:00:00

abstract：:Insect chitinases are a multigene family that is encoded by a rather large and diverse group of genes. The main function of chitinases is to digest the chitin contained in tissues such as the cuticles and gut lining during molting. In this study, we examined the role of a chitinase (SeChi) and a bacterial type chitina...

journal_title：Archives of insect biochemistry and physiology

authors： Zhang D,Chen J,Yao Q,Pan Z,Chen J,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Extracellular signal-regulated kinase (ERK), a mitogen-activated protein kinase (MAPK), plays roles in a variety of cellular responses. However, limited information is available on the relationship between ERKs and environmental stresses. In this report, an ERK gene, AccERK2, was cloned and characterized from Apis cer...

journal_title：Archives of insect biochemistry and physiology

authors： Li Y,Zhang L,Kang M,Guo X,Baohua Xu

更新日期：2012-03-01 00:00:00

abstract：:Antioxidant system, which is composed of multiple gene families, plays a major role in reducing oxidative damage and xenobiotic detoxification in all living organisms. We identified 50 silkworm antioxidant genes from nine gene families based on the assembled genome sequence. A comparative analysis of the antioxidant g...

journal_title：Archives of insect biochemistry and physiology

authors： Shi GQ,Yu QY,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...

journal_title：Archives of insect biochemistry and physiology

authors： Aurade RM,Jayalakshmi SK,Udikeri SS,Sreeramulu K

更新日期：2012-01-01 00:00:00

abstract：:At 22°C and under a long-day photoperiod of L:D 16:8, all the last fifth instar Loxostege sticticalis larvae undergo prepupal stage and pupate without diapause. Under a short-day photoperiod of L:D 12:12, in contrast, they all enter diapause with approximately 36 days diapause maintenance and then terminate diapause s...

journal_title：Archives of insect biochemistry and physiology

authors： Jiang X,Huang S,Luo L

更新日期：2011-07-01 00:00:00

abstract：:This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...

journal_title：Archives of insect biochemistry and physiology

authors： Mesquita-Rodrigues C,Saboia-Vahia L,Cuervo P,Levy CM,Honorio NA,Domont GB,de Jesus JB

更新日期：2011-04-01 00:00:00

abstract：:Chitin synthase catalyzes chitin synthesis in the exoskeleton, tracheal system and gut during insect development. A chitin synthase 1 (CfCHS1) cDNA was identified and cloned from the spruce budworm, Choristoneura fumiferana. The CfCHS1 cDNA is 5,300 bp in length and codes a 1,564-amino acid protein with a molecular ma...

journal_title：Archives of insect biochemistry and physiology

authors： Ampasala DR,Zheng S,Zhang D,Ladd T,Doucet D,Krell PJ,Retnakaran A,Feng Q

更新日期：2011-02-01 00:00:00

abstract：:The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...

journal_title：Archives of insect biochemistry and physiology

authors： Matthews HJ,Down RE,Audsley N

更新日期：2010-11-01 00:00:00

abstract：:Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreove...

journal_title：Archives of insect biochemistry and physiology

authors： Meng F,Zhang L,Kang M,Guo X,Xu B

更新日期：2010-10-01 00:00:00

abstract：:We cloned a full-length tyrosine hydroxylase cDNA from the integument of the diamondback moth, Plutella xylostella. In the phylogenetic tree, tyrosine hydroxylase (PxTH) clustered with the other lepidopteran THs. Serine residues in the PxTH sequence, namely Ser(24), Ser(31), Ser(35), Ser(53), and Ser(65), were predict...

journal_title：Archives of insect biochemistry and physiology

authors： Hwang SH,Cho S,Park YC

更新日期：2010-10-01 00:00:00

abstract：:The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G p...

journal_title：Archives of insect biochemistry and physiology

authors： Cheng Y,Luo L,Jiang X,Zhang L,Niu C

更新日期：2010-09-01 00:00:00

abstract：:When diapause and non-diapause eggs of the same bivoltine strain of Bombyx mori were chilled at 5 degrees C for more than 30 days, the hatchability of diapause eggs increased while that of non-diapause eggs decreased, respectively. To investigate the relationship between effects of chilling on the hatchability and the...

journal_title：Archives of insect biochemistry and physiology

authors： Zhao LC,Shi LG

更新日期：2010-06-01 00:00:00

abstract：:Series of agonists and antagonists were examined for their actions on a Bombyx morialpha-adrenergic-like octopamine receptor (OAR) stably expressed in HEK-293 cells. The rank order of potency of the agonists was clonidine>naphazoline>tolazoline in Ca(2+) mobilization assays, and that of the antagonists was chlorpromaz...

journal_title：Archives of insect biochemistry and physiology

authors： Huang J,Hamasaki T,Ozoe Y

更新日期：2010-02-01 00:00:00

abstract：:There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for...

journal_title：Archives of insect biochemistry and physiology

authors： Lwalaba D,Hoffmann KH,Woodring J

更新日期：2010-01-01 00:00:00

abstract：:A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanoga...

journal_title：Archives of insect biochemistry and physiology

authors： Sonoda S,Tsumuki H

更新日期：2010-01-01 00:00:00

abstract：:Molting in crustaceans is induced by ecdysteroids as in insects. The ecdysteroid titre in hemolymph is negatively regulated by molt-inhibiting hormone (MIH) that inhibits the secretion of ecdysteroids from the Y-organ, an ecdysteroid-producing gland of crustaceans, whereas little is known about the molecular mechanism...

journal_title：Archives of insect biochemistry and physiology

authors： Asazuma H,Nagata S,Nagasawa H

更新日期：2009-12-01 00:00:00

abstract：:To determine differential gene expression profiles in the venom gland and sac (gland/sac) of a solitary hunting wasp species, Orancistrocerus drewseni Saussure (1857), a subtractive cDNA library was constructed by suppression subtractive hybridization. A total of 498 expressed sequence tags (EST) were clustered and as...

journal_title：Archives of insect biochemistry and physiology

authors： Baek JH,Woo TH,Kim CB,Park JH,Kim H,Lee S,Lee SH

更新日期：2009-08-01 00:00:00

abstract：:Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic activity of its tran...

journal_title：Archives of insect biochemistry and physiology

authors： Shi M,Huang F,Chen YF,Meng XF,Chen XX

更新日期：2009-01-01 00:00:00

abstract：:We have employed the proteomic approach in combination with mass spectrometry to study the immune response of honey bee workers at different developmental stages. Analysis of the hemolymph proteins of noninfected, mock-infected and immune-challenged individuals by polyacrylamide gel electrophoresis showed differences ...

journal_title：Archives of insect biochemistry and physiology

authors： Randolt K,Gimple O,Geissendörfer J,Reinders J,Prusko C,Mueller MJ,Albert S,Tautz J,Beier H

更新日期：2008-12-01 00:00:00

abstract：:Bursicon is a neuropeptide that regulates cuticle sclerotization (hardening and tanning) via a G protein-coupled receptor. It consists of two subunits, an alpha and a beta. In the present study, we investigated the transcriptional expression and in situ localization of bursicon alpha and beta in the central nerve syst...

journal_title：Archives of insect biochemistry and physiology

authors： Wang S,An S,Song Q

更新日期：2008-06-01 00:00:00

abstract：:The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

journal_title：Archives of insect biochemistry and physiology

authors： Zhang L,Luo L,Jiang X

更新日期：2008-06-01 00:00:00

abstract：:A major long-term goal of polydnavirus (PDV) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. As PDV viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be ...

journal_title：Archives of insect biochemistry and physiology

authors： Rodríguez-Pérez MA,Beckage NE

更新日期：2008-04-01 00:00:00

abstract：:Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...

journal_title：Archives of insect biochemistry and physiology

authors： Vieira CU,Bonetti AM,Simões ZL,Maranhão AQ,Costa CS,Costa MC,Siquieroli AC,Nunes FM

更新日期：2008-02-01 00:00:00

abstract：:Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...

journal_title：Archives of insect biochemistry and physiology

authors： Tavva VS,Palli SR,Dinkins RD,Collins GB

更新日期：2007-07-01 00:00:00

abstract：:Larvae of Sesamia nonagrioides developing under long day (LD) conditions pupate in the 5th or 6th instar, whereas under the short day (SD) conditions, they undergo several supernumerary larval molts and are regarded as diapausing. The development in early larval instars occurs in the LD larvae at a moderate and in the...

journal_title：Archives of insect biochemistry and physiology

authors： Eizaguirre M,López C,Schafellner C,Sehnal F

更新日期：2007-06-01 00:00:00

abstract：:Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...

journal_title：Archives of insect biochemistry and physiology

authors： García-Gil De Muñoz F,Lanz-Mendoza H,Hernández-Hernández FC

更新日期：2007-05-01 00:00:00

abstract：:Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...

journal_title：Archives of insect biochemistry and physiology

authors： Robertson SL,Ni W,Dhadialla TS,Nisbet AJ,McCusker C,Ley SV,Mordue W,Mordue 'Luntz' AJ

更新日期：2007-04-01 00:00:00

abstract：:Bostrichiformia is the less known major series of Coleoptera regarding digestive physiology. The midgut of Dermestes maculatus has a cylindrical ventriculus with anterior caeca. There is no cell differentiation along the ventriculus, except for the predominance of cells undergoing apocrine secretion in the anterior re...

journal_title：Archives of insect biochemistry and physiology

authors： Caldeira W,Dias AB,Terra WR,Ribeiro AF

更新日期：2007-01-01 00:00:00

abstract：:The glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol (DIA) was used to investigate the action of hypertrehalosemic hormone (HTH) on carbohydrate, neutral lipid, and phospholipid metabolism in the hemolymph and fat body of the cockroach, Periplaneta americana. DIA blocked the hypertrehalosemic and hyperglycemic...

journal_title：Archives of insect biochemistry and physiology

authors： Oguri E,Steele JE

更新日期：2006-12-01 00:00:00

abstract：:Male German cockroaches possess secretory glands that secrete fluid into a pair of transverse depressions on the seventh and eighth abdominal tergites. We investigated the effects of altered juvenoid titer during the first part of the last instar on tergal gland secretory tissue development and the production of terga...

journal_title：Archives of insect biochemistry and physiology

authors： Saltzmann KA,Saltzmann KD,Neal JJ,Scharf ME,Bennett GW

更新日期：2006-09-01 00:00:00

abstract：:The relatively little-investigated entomopathogen Conidiobolus coronatus secretes several proteinases into culture broth. Using a combination of ion-exchange and size-exclusion chromatography, we purified to homogeneity a serine proteinase of Mr 30,000-32,000, as ascertained by SDS-PAGE. The purified enzyme showed sub...

journal_title：Archives of insect biochemistry and physiology

authors： Bania J,Samborski J,Bogus M,Polanowski A

更新日期：2006-08-01 00:00:00

abstract：:Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...

journal_title：Archives of insect biochemistry and physiology

authors： Frantsevich L,Gorb S

更新日期：2006-05-01 00:00:00

abstract：:Two blue-pigment binding proteins, BP1 and BP2, are present in larval and pupal haemolymph of cabbage white butterfly, Pieris rapae, and fluctuate in expression during development. Both BP1 and BP2 are found in pupal haemolymph in varying proportions as well as in adult haemolymph, while only small amounts of BP2 are ...

journal_title：Archives of insect biochemistry and physiology

authors： Choi CW,Nam KP,Seo DH,Choi JW,Lee CS,Kim HR,Yun CY

更新日期：2006-04-01 00:00:00

abstract：:Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...

journal_title：Archives of insect biochemistry and physiology

authors： Harvey JA,Vet LE,Witjes LM,Bezemer TM

更新日期：2006-03-01 00:00:00

abstract：:The biological activity of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was examined in vivo toward larvae and pupae of Galleriae mellonella L. (Lepidoptera: Pyralidae), and in vitro toward bacterial and fungal cultures, as well as cultured insect cells. Pupae of G. mellonella were far more susceptibl...

journal_title：Archives of insect biochemistry and physiology

authors： Ergin E,Uçkan F,Rivers DB,Sak O

更新日期：2006-02-01 00:00:00

abstract：:Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...

journal_title：Archives of insect biochemistry and physiology

authors： Puttaraju HP,Prakash BM

更新日期：2005-12-01 00:00:00

abstract：:Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves...

journal_title：Archives of insect biochemistry and physiology

authors： Barbehenn R,Dodick T,Poopat U,Spencer B

更新日期：2005-09-01 00:00:00

abstract：:Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated ...

journal_title：Archives of insect biochemistry and physiology

authors： Blenau W,Baumann A

更新日期：2005-07-01 00:00:00